What is Regan-Lowe agar selective for?
By Mia Lopez
What is Regan-Lowe agar selective for?
INTRODUCTION. Regan-Lowe Charcoal Agar is a selective medium used for the isolation of Bordetella pertussis from clinical specimens.
What is Regan-Lowe media?
Our Regan-Lowe Transport Medium is a semi- solid medium used to transport clinical specimens containing Bordetella species. Niacin or nicotinic acid is incorporated into the medium since Proom determined it to be an essential growth factor for some Bordetella species.
Is Bordet Gengou agar selective or differential?
Bordet-Gengou Agar with Charcoal is a selective medium used for the isolation of Bordetella pertussis from clinical specimens. The medium is used in clinical laboratories for the isolation of Bordetella pertussis, the etiologic agent of whooping cough, from nasopharyngeal swabs and other sources of pharyngeal exudate.
What is the purpose of Bordet Gengou Agar?
Bordet Gengou Agar is an enriched casein peptone medium with potato infusion and glycerol to supply nutrients which support the growth of Bordetella spp. Blood is added to provide additional nutrients and to enable the detection of hemolytic reactions. Sodium chloride maintains osmotic equilibrium.
Is Bordetella a pertussis?
Pertussis, a respiratory illness commonly known as whooping cough, is a very contagious disease caused by a type of bacteria called Bordetella pertussis. These bacteria attach to the cilia (tiny, hair-like extensions) that line part of the upper respiratory system.
Which is the highly infective stage in whooping cough?
People with pertussis are most infectious during the catarrhal period and during the first two weeks after onset of the cough (approximately 21 days).
What agar does Bordetella pertussis grow on?
Bordetella pertussis pertussis may grow on Bordetella selective medium (charcoal blood agar with cefalexin) within three days, but normally 5-7 days incubation is required for primary isolation. Plates should be incubated for 7 days before being discarded as negative6.
What grows on Brucella agar?
Brucella Agar is a general purpose medium for the cultivation of Streptococcus pneumoniae, Streptococcus viridans, and Neisseria meningitidis. With the addition of blood, Brucella Agar is used to determine bacterial hemolytic reactions.
How is whooping cough caused?
Why does pertussis cause lymphocytosis?
Pertussis toxin (PT), from Bordetella pertussis, causes lymphocytosis and increased IL-4 and IgE secretion. The lymphocytosis is associated with impaired entry of lymphocytes into lymph nodes. The dose response of PT on IL-4 secretion was found to be similar to those for lymphocytosis and IgE production.
How long is the incubation period for pertussis?
The incubation period of pertussis is commonly 7 through 10 days, with a range of 4 through 21 days. The clinical course of the illness is divided into three stages: catarrhal, paroxysmal, and convalescent.
