What is l-15 Medium?
Leibovitz’s L-15 Medium supports the growth of HEP-2 monkey kidney cells and primary explants of embryonic and adult human tissue. L-15 is buffered by phosphates and free base amino acids instead of sodium bicarbonate. This medium is designed for supporting cell growth in environments without CO2 equilibration.
What is l-15?
L-15 Medium (Leibovitz) was originally formulated for use in carbon dioxide (CO2) free systems requiring sodium bicarbonate. L-15 is buffered by the use of free basic amino acids, phosphate buffers and higher levels of galactose and sodium pyruvate to help maintain physiological pH control.
What is in RPMI medium?
RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle’s Minimal Essential Medium or Dulbecco’s Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors.
What is Xeno-free medium?
Description. Xeno-free and chemically-defined medium devoid of animal- or human-derived components. For stem cell expansion or other uses that must avoid variability due to undefined animal components.
What is Xeno-free culture?
It is my understanding that xeno-free is defined as all components (i.e. in the cell culture medium) are derived from the same organism whether it is animal (e.g. bovine) or human. These products could help us reach our goal of the ideal stem cell media – devoid of any animal or human derived components.
Can I use DMEM instead of RPMI?
On a very basic level in our laboratory you would use DMEM for an adherent cell line and you would use RPMI for suspension cell lines. For example some cell lines grow and flourish better in the presence of HEPEs. Also you need to think about your down the line uses for the cells.
What is serum-free media?
Serum-free media are media designed to grow a specific cell type or perform a specific application in the absence of serum. The use of serum-free media (SFM) represents an important tool, that allows cell culture to be done with a defined set of conditions as free as possible of confounding variables.
