What is GFP antibody?
By Gabriel Cooper
What is GFP antibody?
GFP is a versatile marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression. All our anti-GFP antibody conjugates are suited for detection of native GFP, GFP variants, and most GFP fusion proteins by western blot analysis and immunocytochemistry.
What is the isoelectric point of GFP?
U57607) is a protein composed of 293 amino acid residues (32.7 kDa) that has an isoelectric point of 6.2 and exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range.
What is the molecular weight of RFP?
27 kDa
RFP Control is suitable as a control for polyclonal or monoclonal Anti-RFP in immunological assays. The molecular weight of RFP (calculated) is 27 kDa, but in a gel it is expected to run at approximately 28-30 kDa.
What is the molecular weight of BFP?
29.0 kDa
Product Details
| Alternate Name | BFP, Blue Fluorescent Protein |
|---|---|
| Source | E. coli |
| Appearance | Lyophilized protein |
| Physical Form Description | Freeze Dried |
| Molecular Weight | 29.0 kDa |
What is GFP and RFP?
Green Fluorescent Protein (GFP) and Red Fluorescent Protein (RFP) are ~29 kDa fluorescent proteins used as versatile markers for visualization of protein localization, monitoring physiological processes, and for detecting transgenic or transient protein expression.
What is the difference between GFP and RFP?
The RFP emission filter permits signals with 575 nm to 640 nm to pass through and the GFP emission filter permits fluorescent signals with wavelengths of 510 nm to 523 nm to pass through.
Why is GFP classified as a tertiary protein?
Since the side chains are sticking out, they can interact with one another, leading to “tertiary structure.” GFP has a lot of residues which like water (are hydrophilic) and a lot of others which want to avoid it (are hydrophobic).
How many alpha helices are in GFP?
6 alpha helices
Wild type GFP contains 238 amino acids, folded into a series of 6 alpha helices and 11 beta strands, connected by loops.
What does the GFP antibody detect?
Specificity / Sensitivity. GFP Antibody detects GFP, YFP, and CFP-tagged proteins exogenously expressed in cells. This antibody does not detect RFP-tagged proteins. Please note that the GFP, YFP, and CFP tags add approximately 27 kDa to the molecular weight of the fusion protein.
What is green fluorescent protein (GFP)?
Green fluorescent protein (GFP) is a β-barrel-shaped protein 1 consisting of 238 amino acids and with a molecular weight of ~27kDa. GFP is isolated from the crystal jellyfish Aequorea victoria, and its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light via energy transfer2.
What is the role of GFP in cell culture?
GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted.
What is the function of GFP tag in jellyfish?
The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms.
